Interspecific and intergeneric hybridization between genetically engineered canola (Brassica napus L.) and related weed species.

摘要

A complete diallel cross was constructed between 3 weedy species, field mustard (B. rapa [B. campestris]), wild mustard (B. kaber) and black mustard (B. nigra), and canola [rape] genetically engineered for resistance to glufosinate [via a phosphinothricin acetyltransferase gene]. Samples of developing pods were collected from each possible cross combination at 4-day intervals from 2 to 26 days after pollination. The percentage of developing ovules was highest in the canola X field mustard cross andits reciprocal cross (81.1 and 74.5%, respectively). Hybrid seeds were obtained from field mustard X canola crosses without the use of tissue culture or embryo rescue. Hybrid plants were sprayed twice with glufosinate to check for resistance. All hybrids involving the glufosinate-resistant canola as one of the parents were resistant, indicating that the herbicide resistance trait was transferred to hybrid progeny. Most hybrids were sterile, but a few produced self-fertilized seeds.