Synthesis and characterization of a amphiphilic pluronic-poly(D,L-lactide-co-glycolide) copolymer and their nanoparticles as protein delivery systems

摘要

A new amphiphilic Pluronic (F68)-PLGA copolymer, which can be used to prepare the stealth or long-circulating nanoparticles, was synthesized with Pluronic (F68), DL-Lactide, and glycolide. The structures of Pluronic (F68)-PLGA copolymer as the products Were characterized with infrared spectrometry, nuclear magnetic resonance and their molecular weights were determined by gel permeation chromatography. TWO methods, double emulsion (DE) and nanoprecipitation (NP), were employed to fabricate the polymeric nanoparticles. Bovine serum albumin (BSA) was loaded into nanoparticles as a model protein. Influence of the preparation conditions on the nanoparticles size, encapsulation efficiency, and release profile in vitro was investigated. They showed the entrap-ment efficiency of 42.9-63.4% and the average diameter of 119.1-342.8 nm depending on the fabrication technique of nanoparticles and the type of copolymer. The stability maintenance of BSA in the nanoparticle release in vitro was also measured via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), circular dichroism (CD), and fluorescence spectrometry. The results showed that the new Copolymer could load BSA effectively and BSA kept stable after it was released from the nanoparticles. (c) 2008 Wiley Periodicals, Inc.