Preparation and characterization of an enzyme electrode based on cholesterol esterase and cholesterol oxidase immobilized onto conducting polypyrrole films

摘要

Cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) enzymes were entrapped within polypyrrole (PPy) films on a platinum disc electrode during electro-chemical polymerization. The characteristics of the PPy/ChEt/ChOx enzyme electrode thus prepared were investigated as a function of the time, pH, temperature, and concentration of cholesteryl palmitate by a spectrophotometric method. PPy/ChEt/ChOx electrodes can be used for the estimation of cholesteryl palmitate concentrations from I to 8 mM, can be used least 10 times, and have a shelf life of about I month at 4-10degreesC. (C) 2004 Wiley Periodicals, Inc. [References: 30]