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Characterization of Three Glucosyltransferase Genes in Tartary Buckwheat and Their Expression after Cold Stress

机译:苦荞中三个葡萄糖基转移酶基因的鉴定及其在冷胁迫下的表达

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摘要

Anthocyanins confer the red color in the hypocotyl of tartary buckwheat sprouts. Uridine diphosphate (UDP)-glucose:flavonoid 3-O-glycosyltransferase (UFGT) stabilizes anthocyanin by attaching the glucosyl moiety from UDP-glucose to the C-3 hydroxyl of anthocyanin. In this study, we characterized three UFGT-like genes, designated FtUFGT1, 2, and 3 from tartary buckwheat. The results revealed that FtUFGT1, FtUFGT2, and FtUFGT3 can convert cyanidin to cyanidin 3-O-glucoside, with specific activities of 20.01 X 10(-3), 8.93 X 10(-3), and 20.24 X 10(-3) IU/mg, respectively. The active-site residues of the C-terminal domains and the N-terminal domains are important for the donor and acceptor recognition of these proteins. The expression of the three FtUFGTs paralleled the tissue-specific anthocyanin accumulation. After cold treatment, the increased content of anthocyanin was accompanied by the up-regulated expression of the three FtUFGTs. Among these three UGFT gene members, FtUFGT3 showed the highest expression level and the highest specific activity, suggesting that FtUFGT3 might be the major gene involved in anthocyanin biosynthesis. These results suggested that the FtUFGT genes, FtUFGT3 in particular, might be important candidates for anthocyanin formation in tartary buckwheat sprouts.
机译:花青素使苦荞芽的下胚轴呈红色。尿苷二磷酸(UDP)-葡萄糖:类黄酮3-O-糖基转移酶(UFGT)通过将UDP-葡萄糖的葡萄糖基部分连接到花青素的C-3羟基上来稳定花青素。在这项研究中,我们表征了三个UFGT样基因,分别是来自苦荞的FtUFGT1、2和3。结果显示FtUFGT1,FtUFGT2和FtUFGT3可以将花青素转化为花青素3-O-葡萄糖苷,比活度分别为20.01 X 10(-3),8.93 X 10(-3)和20.24 X 10(-3)IU / mg。 C端结构域和N端结构域的活性位点残基对于这些蛋白质的供体和受体识别很重要。这三个FtUFGT的表达与组织特异性花色苷的积累平行。经过冷处理后,花青素含量的增加伴随着三种FtUFGTs的表达上调。在这三个UGFT基因成员中,FtUFGT3表现出最高的表达水平和最高的比活性,表明FtUFGT3可能是花色苷生物合成中涉及的主要基因。这些结果表明,FtUFGT基因,特别是FtUFGT3,可能是苦荞芽中花青素形成的重要候选者。

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