In Vitro Fermentation of Xylooligosaccharides Produced from Miscanthus x giganteus by Human Fecal Microbiota

摘要

Purified xylooligosaccharides from Miscanthus X giganteus (MxG XOS) were used in an in vitro fermentation experiment inoculated with human fecal microbiota. A commercial XOS product and pectin were used as controls. Decreases in pH by 2.3, 2.4, and 2.0 units and production of short-chain fatty acids (SCFA; acetic acid, 7764.2, 6664.1, and 6387.9 mu mol/g; propionic acid, 1006.7, 1089.5, and 661.5 mu mol/g; and butyric acid, 955.5, 1252.9, and 917.7 mu mol/g) were observed in MxG XOS, commercial XOS, and pectin medium after 12 h of fermentation, respectively. Titers of Bifidobacterium spp., Lactobacillus spp., and Escherichia coli increased when fed all three substrates as monitored by qPCR. There was no significant trend for Clostridium perfringens. During fermentation, MxG XOS was statistically equivalent in performance to the commercial XOS sample as measured by culture acidification and growth of health-promoting bacteria and resulted in the highest SCFA production among the three substrates.