Inbred Chinese Wuzhishan (WZS) Minipig Model for Soybean Glycinin and β-Conglycinin Allergy

摘要

Validated murine models have been built to assess the potential allergenicity of novel proteins. Large animals, such as pigs, share more similarities to humans in physiology and immunology than murine. Among Chinese minipigs, Wuzhishan (WZS) minipigs have the highest inbreeding coefficient, more stable heredity, and less variability, which were gastrically sensitized and excitated with diets containing 4% glycinin or 4% β-conglycinin or neither to induce anaphylactic reactions in the present study. In glycinin- and β-conglycinin-sensitized animals, diarrhea symptoms and skin wheal and flare responses were observed. In comparison to the control, after oral excitation with glycinin or β-conglycinin, the serum IgE was increased by 34.4 or 38.4% and the serum histamine was increased by 42.1 or 46.9%, respectively. In addition, the serum IFN-γ were reduced by 12.4 or 30.0%, respectively. The jejunum histamine level of β-conglycinin-sensitized animals was increased by 196.6%, while the number of mast cells in the submucosa of jejunum and ileum of the glycinin-sensitized animals declined by 48.1 and 45.0%. In conclusion, the WZS minipig allergy models induced by soybean glycinin and β-conglycinin represent type-l hypersensitivity reactions mediated by IgE, which could potentially be useful in determining the potential allergenicity of novel proteins.