Metabolic Profiling of Turmeric(Curcuma Ionga L.)Plants Derived from in Vitro Micropropagation and Conventional Greenhouse Cultivation

摘要

Turmeric(Curcuma longa)was considered only a culinary spice in many parts of the world until the notable anti-inflammation curcuminoids were discovered from this herb.Because it is a sterile triploid and is propagated vegetatively by rhizome division,turmeric is susceptible to pathogens that accumulate and are transmitted from generation to generation,and amplification of particularly useful stocks is a slow process.An in vitro propagation method has been developed to alleviate these problems.Metabolic profiling,using GC-MS and LC-ESI-MS,was used to determine if chemical differences existed between greenhouse-grown and in vitro micropropagation derived plants.The major chemical constituent curcuminoids,a group of diarylheptanoid,as well as major mono-and sesquiterpenoids were identified and quantified.Principal component analysis and hierarchical cluster analysis revealed chemical differences between lines(T3C turmeric vs Hawaiian red turmeric)and tissues(rhizome,root,leaf,and shoot).However,this analysis indicated that no significant differences existed between growth treatments(conventional greenhouse-grown vs in vitro propagation derived plants).