Isolation and Characterization of a Novel Immunomodulatory alpha-Glucan Protein Complex from the Mycelium of Tricholoma matsutake in Basidiomycetes

摘要

Tricholoma matsutake,a high-class edible mushroom in Japan,has been reported to have excellent biological activities,but difficulty in cultivating the fruit bodies and limited bulk availability have restricted detailed studies.We have developed a method of culturing in tanks,enabling the bulk supply of the mycelia.The preparation(CM6271)exerts modulative effects on the immune competence of mice and rats.In this study,a sodium hydroxide extract of CM6271 was defatted followed by fractionation with a combination of ion exchange chromatography and gel filtration in order to identify the components involved in the expression of the activity,and a single peak fraction(MPG-1)was obtained with reversed phase chromatography.MPG-1 was a glycoprotein(suganprotein ratio,94.3:5.7)with a relative molecular mass of 360 kDa,and the sugar moiety contained about 90% glucose.NMR spectra and methylation analysis revealed that the alpha-1,4-linkage was the predominant glucan linkage with alpha-1,6-and alpha-1,2-linkages in the minority.The amino acid composition in the protein moiety was rich in glutamine,alanine,asparagine,leucine,glycine,valine,serine,threonine,isoleucine,and proline.MPG-1 was resistant to degradation with amylase or protease.The oral administration of MPG-1 promoted,in a dose-dependent manner,the recovery of the mouse natural killer cell activity and serum IL-12 level that had been reduced by the loading of restraint stress.The dose of MPG-1(25 mg/kg)required for the expression of the effect decreases to 1/12 of that of CM6271(300 mg/kg).Furthermore,MPG-1 formed a complex with TGF-beta1 in vitro,modulating the biological activity of TGF-beta1 by binding to its active form.These results indicate that the mycelium of 7.matsutake contains a novel alpha-glucan-protein complex with immunomodulatory activities.