Identification of Food-Derived Collagen Peptides in Human Blood after Oral Ingestion of Gelatin Hydrolysates

摘要

In the present study,we identified several food-derived collagen peptides in human blood after oral ingestion of some gelatin hydrolysates.Healthy human volunteers ingested the gelatin hydrolysates(9.4-23 g)from porcine skin,chicken feet,and cartilage after 12 h of fasting.Negligible amounts of the peptide form of hydroxyproline(Hyp)were observed in human blood before the ingestion.After the oral ingestion,the peptide form of Hyp significantly increased and reached a maximum level(20-60 nmol/mL of plasma)after 1-2 h and then decreased to half of the maximum level at 4 h after the ingestion.Major constituents of food-derived collagen peptides in human serum and plasma were identified as Pro-Hyp.In addition,small but significant amounts of Ala-Hyp,Ala-Hyp-Gly,Pro-Hyp-Gly,Leu-Hyp,lie-Hyp,and Phe-Hyp were contained.