Open Pulled Straw (OPS) Vitrification of Porcine Blastocysts: Simple Procedure Yielding Excellent in Vitro Survival. But so far no Pigets Following Transfer

摘要

In 2 trials, 346 porcine in vivo blastocysts were vitrified in heat-softened and pulled French straws by the Open Pulled Straw (OPS)vitrification method. After thawing, embryos were either cultured in vitro (n=89) for 24 to 48 hours or transferred surgically (n=257) to 11 synchronised recipients without further culture. In trial 1,88 precent and 71 precent of thawed blastocysts reexpanded and hatched in vitro, respectively, while 71 precent reexpanded in trial 2. However, no piglets were born following transfer, though 1 recipient was diagnosed pregnant at Day 56 of gestation.