BOTH DEATH RECEPTOR AND MITOCHONDRIA MEDIATED APOPTOTIC PATHWAYS PARTICIPATED THE OCCURRENCE OF APOPTOSIS IN PORCINE VITRIFIED MII STAGE OOCYTES

摘要

BACKGROUND: Oocytes vitrification is widely used for cryopreservation of female genetic resources. OBJECTIVE: In order to illuminate the apoptotic pathways of porcine MII stage oocytes after vitrification. MATERIALS AND METHODS: This study used in situ fluorescence staining and RT-PCR to detect the expression levels of some key molecules from death receptor and mitochondria mediated apoptotic pathways. RESULTS: (1) Early stage apoptosis were detected in both PI staining survival oocytes and PI staining dead oocytes. (2) The fluorescence intensity of caspase 8, caspase 9, caspase 3 and pan caspase from vitrified oocytes were 32.03, 16.56, 16.70 and 8.43 respectively, which were much higher than those from fresh oocytes (4.02, 4.83, 4.23 and 3.08, P < 0.05). (3) Not only the genes from death receptor mediated apoptotic pathway, but also from mitochondrial mediated apoptotic pathway were changed greatly. CONCLUSION: The death of porcine vitrified oocytes could be induced by apoptosis, both death receptor and mitochondria mediated apoptotic pathways participated the occurrence of apoptosis in porcine vitrified MII stage oocytes.