Effect of Cell Density and HLA-DR Incompatibility on T-Cell Proliferation and Forkhead Box P3 Expression in Human Mixed Lymphocyte Reaction

摘要

Background. The proliferation rates of human T cells in vitro are affected by some factors such as initial T-cell number, dose of stimulating cells, and duration of culture. The transcription factor forkhead box P3 (FoxP3) has been used to identify regulatory T cells in humans and is thought to correlate with tolerance to allogeneic organ transplant. Thus, it is important to optimize conditions to expand FoxP3 cell proliferation to improve engraftment of allogeneic organ transplants.