Transient Excited-State Absorption and Gain Spectroscopy of a Two-Photon Absorbing Probe with Efficient Superfluorescent Properties

摘要

The synthesis, linear photophysical properties, two-photon absorption (2PA), excited-state transient absorption, and gain spectroscopy of a new fluorene derivative tert-butyl 4,4'-(4,4' (lE,l'E)-2,2'-(9,9-bis(2-(2-ethoxyethoxy)-ethyl)-9H-fluorene-2,7-diyl)bis(ethene-2,l-diyl)bis(4,1 phenylene)]dipiperazine-l-carboxylate (1) are reported. The steady-state linear absorption and fluorescence spectra along with excitation anisotropy, fluorescence lifetimes, and photochemical stability of 1 were investigated in a number of organicsolvents at room temperature. The 2PA spectra of 1 with a maximum cross section of ~300 GM were obtained with a 1-kHz femtosecond laser system using open-aperture Z-scan and two-photon-induced fluorescence methods. The transient excited-state absorption (ESA) and gain kinetics of 1 were investigated by a femtosecond pump-probe methodology. Fast relaxation processes (~1-2 ps) in the gain and ESA spectra of 1 were revealed in ACN solution, attributable to symmetry-breaking effects in the first excited state. Efficient superfluorescence properties of 1 were observed in a nonpolar solvent under femtosecond excitation. One- and two-photon fluorescence microscopy imaging of HCT 116 cells incubated with probe 1 was accomplished, suggesting the potential of this new probe in two-photon fluorescence microscopy bioimaging.