A Set of New Simple Sequence Repeat and Avenin DNA Markers Suitable for Mapping and Fingerprinting Studies in Oat (Avena spp.)

摘要

This study was conducted to develop and test new markers for use in molecular characterization of oat (Avena spp.) Results are presented for two polymerase chain reaction (PCR)-based markers targeting multiple avenin protein loci and 16 new microsatellite or simple sequence repeat (SSR) markers. The markers were mapped in two segregating oat (Avena sativa L.) populations, 'Kanota' x 'Ogle' (KxO) and 'Terra' x 'Marion' (TxM), and were also tested across a diversity panel consisting of 35 cultivated varieties of oat and representatives of five other Avena species. All markers were robust, multi-allelic, and highly polymorphic. The SSR markers, some highlighting multiple loci, mapped throughout the genome, and the avenin markers mapped to their expected locations in KxO and TxM. Also as expected, the avenin markers were shown to be linked to the stem rust resistance gene Pg9 in the population 'OT328' x 'Dumont' (OTxDu). Practical applications in variety identification and germplasm diagnostic assays were explored. The markers were able to distinguish closely related lines for fingerprinting purposes and were also useful for determining whether variation within a line was the result of residual heterozygosity or contamination. In addition, the markers were found to be useful for forensic purposes and for determining whether crossing and backcrossing had been successful.