Effect of different inclusion levels of Morus alba Linn cv. Cubana on the methane fermentation and production under in vitro conditions with rumen liquor from river buffaloes (Bubalus bubalis)

摘要

In order to evaluate the effect of different inclusion levels of mulberry (A/, alba Linn.) cv. Cubana on the fermentation and reduction of the rumen methanogenesis in river buffaloes {Bubalus bubalis), an in vitro fermentation was performed and five levels of inclusion of hi. alba L. cv. Cubana (0. 15, 20, 25 y 30 %) were assessed in a diet based on star grass (Cynodon nlemfuensis). The volume of gas and methane accumulated was determined at 4, 8, 12, and 24 h of fermentation, as well as the concentration of total and individual short chain fatly acids (SCFA), the ammonia (Nil.), and the pll at 0, 4, 8. 12, and 24 h of incubation. The inclusion of M. alba L. cv. Cubana increased the production of gas accumulated at 24 h of fermentation, compared with the treatment containing only star grass. The different mulberry inclusion levels reduced the methane production in the rumen, compared with the control. The 30 % was the level that reduced it the most The concentration of total and individual SCFA wasnot affected, nor that of Nil,. It was concluded that the inclusion levels of 15, 20, 25, and 30 % of M. alba L. cv. Cubana did not affect the end products of ihe fermentation and reduced the in vitro rumen methane production. The 30 % was the level that reduced the most the rumen methanogenesis.