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Evaluation of Brazilian sugarcane genotypes for resistance to Sugarcane mosaic virus under greenhouse and field conditions

机译:在温室和田间条件下评估巴西甘蔗基因型对甘蔗花叶病毒的抗性

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This study aimed to evaluate the resistance of 79 sugarcane genotypes (varieties and elite clones) artificially inoculated with a severe Sugarcane mosaic virus (SCMV) strain and to estimate genetic parameters associated with SCMV resistance. Symptoms were evaluated using a graded scale, and confirmation of infection was performed using the Plate Trapped Antibody-ELISA serological test in plants conducted in a greenhouse experiment and then validated under field conditions. The mean incidence of mosaic disease was low in greenhouse conditions, with higher observed incidence values when assessed in the field. Based on the field results, variance analysis was significant for genotypes and genotype x environment interaction. Tracking mosaic symptom expression over time in the different sugarcane genotypes revealed varying behavior, including recovery from disease in some genotypes. The broad-sense heritability based on the mean and individual level was 62.18% and 19.37%, respectively. Twenty-two genotypes were observed to be resistant to SCMV. (C) 2015 Elsevier Ltd. All rights reserved.
机译:这项研究旨在评估人工接种严重甘蔗花叶病毒(SCMV)菌株的79种甘蔗基因型(品种和优良克隆)的抗性,并评估与SCMV抗性相关的遗传参数。使用分级量表评估症状,并使用平板捕获抗体-ELISA血清学试验在温室实验中对植物进行感染确认,然后在田间条件下进行验证。在温室条件下,花叶病的平均发病率较低,在野外评估时观察到的发病率较高。根据实地结果,方差分析对于基因型和基因型x环境相互作用具有重要意义。跟踪不同甘蔗基因型中随时间推移的马赛克症状表达显示出不同的行为,包括某些基因型中疾病的恢复。基于平均水平和个体水平的广义遗传力分别为62.18%和19.37%。观察到22种基因型对SCMV具有抗性。 (C)2015 Elsevier Ltd.保留所有权利。

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