Quantitative Chromatin Immunoprecipitation Using the LightCycler~ 480 System

摘要

Antigen processing and presentation play a critical role in immune surveillance. Peptides derived from self, viral, or tumor-associated antigens are generated in the cytoplasm by the proteasome. Transporter associated with antigen processing (TAP), a member of the ATP-binding cassette (ABC) transporter family, functions by transporting these peptides from the cytoplasm to the lumen of the endoplasmic reticulum (ER), where each peptide forms a ternary complex with beta-2 microglobulin (R2m) and major histocompatibility complex (MHC) class I heavy chain. These complexes are then transported to the cell surface and recognized by cytotoxic T lymphocytes (CTLs), which eventually kill cells that present non-self antigens.