Label-Free and Dynamic Monitoring of Cell-Based Assays

摘要

The xCELLigence system from Roche Applied Science,originally invented by the US company ACEA Biosciences,allows for label-free and dynamic monitoring of cell-basedassays. The xCELLigence system utilizes impedance read-out to non-invasively quantify cellular status in real-timeM. The cells are seeded in 96-well microtiter E-Plates withintegrated microelectronic sensor arrays at the bottom ofeach well. Application of a low-voltage (20 mV) AC cur-rent generates an ionic environment inside the wells ofthe E-Plates that is a reflection of the number of cells inthe well, the morphology of the cells, and the strength ofcell attachment (Figure 1). A number of cell-based appli-cations have been developed for the xCELLigence systemincluding: · Cellular quality control · Cell proliferation · Compound-, cell- and virus-mediated cytotoxicity.0 Barrier function · Cell adhesion and spreading · Receptor-mediated signaling To demonstrate the utility of the xCELLigence system forcell-based assays, a subset of these applications are high-lighted in this article.