RNA Isolation from FFPE Tissues - Comparing the Performances of Three Commercial Kits

摘要

In order to compare the performance of the High Pure FFPE RNA Micro Kit with that of two FFPE RNA isolation kits from other manufacturers, total RNA was isolated from sections of mouse mammary tumors with each of the three kits. Yield and integrity of the isolated RNA as well as genomic DNA (gDNA) contamination were assessed. Real-time RT-PCR of the housekeeping gene ACTB was performed to evaluate performance of the kits. In terms of yield and the A260/280 and A260/230 ratios, kits A and B seemed to perfom better than the High Pure FFPE RNA Micro Kit. In terms of real-time RT-PCR sensitivity, however, the High Pure FFPE RNA Micro Kit showed superior results.