Rapid High-Throughput Methylation Analysis Using the LightCycler 480 System

摘要

The LightCycler? 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler? 480 High Resolution Melting Master mix for DNA methylation analysis using the methyl-atlon-sensitive high resolution melting (MS-HRM) methodology. This proved to be a robust platform for performing MS-HRM experiments, allowing rapid and high-throughput studies of DNA methylation.