Efficient Transfection of Meurobiastoma Cell Lines Using FuGENE HD Transfection Reagent

摘要

Efficient transfection of cells is a prerequisite for genetic manipulation. However, many cell lines remain hard to transfect with established protocols, hampering consecutive analysis. Here, we compare three different methods (FuGENE? HD Transfection Reagent, transfection reagent L, and calcium phosphate) for the transfection of neuroblastoma cell lines. We show that FuGENE? HD Transfection Reagent is able to transfect all of these cell lines with high efficiency for further analysis.