Genome Sequencer 20 System: Breakthrough in a Mew Applications Age of Sequencing

摘要

The Genome Sequencer 20 System is an ultra-high-throughput automated DNA sequencing system capable of carrying out and monitoring sequencing reactions in a massively parallel fashion. Since the Genome Sequencer 20 System provides a complete solution for ultra-high-throughput DNA sequencing, an individual researcher can for the first time prepare samples and sequencing reactions, generate sequence reads, and assemble genome sequence data within days.The whole genome sequencing workflow from sample input to data output consists of DNA library preparation, emulsion-based clonal PCR amplification CemPCR), PicoTiterPlate device preparation, sequencing run, and data analysis. The output of a single run is typically 20 x 1OB nucleotides ormore [forthe 70 x 75 mm PicoTiterPlate device) at an average read length of 100 high-quality bases, and multiple runs can be pooled for off-line assembly/mapping. The final consensus sequence is output as a FASTA file, with an associated basecall quality score file.