Resonance light scattering spectroscopy of procyanidin-CPB-DNA ternary system and its potential application

摘要

A new method for the determination of calf thymus DNA at nanogram level was proposed based on the enhanced resonance light scattering (RLS) signals of DNA in the presence of procyanidin and cetylpyridinium bromide dihydrate (CPB). Under the experimental conditions, the RLS intensity of DNA at 291.0 nm was greatly enhanced by procyanidin-CPB at pH 7.0. There was a good linear relationship (r=0.9993) between the enhanced KS intensity (Delta I-RLS) and DNA concentration of 0.0084-3.36 mu g mL(-1). The limit of detection (LOD) was 2.27 ng mL(-1) (3S(0)/S). Three synthetic DNA samples were measured with satisfactory, and the recovery was 102.3-107.2%. (C) 2015 Elsevier B.V. All rights reserved.