Extraction of intrinsic fluorescence from single fiber fluorescence measurements on a turbid medium

摘要

This study utilizes Monte Carlo simulations of single fiber fluorescence to develop an empirical model that corrects for the influence of scattering and absorption on fluorescence intensity (F_(SF)). The model expresses F_(SF) in terms of the reduced scattering coefficient ((mu)_(s)~(')) and absorption coefficient ((mu)_(a)), each determined independently at excitation and emission wavelengths ((lambda)_(x) and (lambda)_(m)), and the fiber diameter (d_(f)). This model returns accurate descriptions (mean residual <6percent) of F_(SF) across a biologically relevant range of (mu)_(s)~(') and (mu)_(a) values and is insensitive to the form of the scattering phase function.