We have developed a new microscopy design that can achieve wide field-of-view (FOV) imaging and yet possesses resolution that is comparable to a conventional microscope. In our design, the sample is illuminated by a holographically projected light-spot grid. We acquire images by translating the sample across the grid and detecting the transmissions. We have built a prototype system with an FOV of 6 mm X 5 mm and acquisition time of 2.5 s. The resolution is fundamentally limited by the spot size--our demonstrated average FWHM spot diameter was 0.74 (mu)m. We demonstrate the prototype by imaging a U.S. Air Force target and a lily anther. This technology is scalable and represents a cost-effective way to implement wide FOV microscopy systems.
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机译：我们开发了一种新的显微镜设计，可以实现宽视场（FOV）成像，并且具有与传统显微镜相当的分辨率。在我们的设计中，样品由全息投影的光斑栅格照明。我们通过在网格上平移样本并检测传输来获取图像。我们建立了一个原型系统，其FOV为6 mm X 5 mm，采集时间为2.5 s。分辨率从根本上受光斑尺寸的限制-我们展示的平均FWHM光斑直径为0.74μm。我们通过对美国空军目标和百合花药进行成像来演示原型。该技术是可扩展的，代表了一种实现宽视野显微镜系统的经济有效的方法。
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