Immobilization of Antibodies on Biosensing Devices by Nanoarrayed Self-Assembled Monolayers

摘要

This work presents an original and straightforward technique for antibody immobilization onto a surface,keeping the antibody in a biologically reactive configuration.Self-assembly of molecular monolayers and plasma-based colloidal lithography were combined to create chemical nanopatterns on the surface of a biosensing device.This technique was employed to create an array of 100 nm wide motifs having a hexagonal 2-D crystalline structure,characterized by COOH-terminated nanospots in a CH_3-terminated matrix.The quality control of the chemical nanopattern was carried out by combining atomic force microscopy,ellipsometry,and contact angle measurements.Enzyme-linked immunosorbent assay experiments were set up showing that the COOH/CH_3 nanopatterned surface constrains the immobilization of the antibodies in a biologically reactive configuration,thus significantly improving the device performances as compared to those of more conventional nonpatterned COOH-terminated or CH_3-terminated surfaces.