Comment on'Direct Electrochemistry and Electrocatalysis of Heme Proteins Entrapped in Agarose Hydrogel Films in Room-Temperature Ionic Liquids'

摘要

Wang et al.state that"The redox potentials for hemoglobin,myoglobin,horseradish peroxidase,cytochrome c,and catalase(in agarose films)were found to be more negative than those in aqueous solutions".The redox potentials reported for HRP,hemoglobin,myoglobin,and cytochrome c are -320 mV,-348 mV,-430 mV,and -522 mV versus Ag| AgCl,respectively,in agarose films and-470mV,-62 mV,-160mV,and 60 mV,respectively,in aqueous solution.Clearly,the statement made by Wang et al.is not correct in the case of HRP;more importantly,the large decreases in the redox potential observed with hemoglobin,myoglobin,and cytochrome c are indicative of substantial structural changes.