Formation of Polyelectrolyte Multilayer Architectures with Embedded DMPC Studied in Situ by Neutron Reflectometry

摘要

The coupling of lipid molecules to polymer components in a planar biomimetic model membrane made of a lipid bilayer(dimyristoylphosphatidylcholine)supported by polyelectrolyte multilayers is studied.The polyelectrolyte support was prepared by layer-by-layer deposition of positively charged poly(allylamine hydrochloride)(PAH)and negatively charged poly(sodium 4-styrenesulfonate)(PSS).Two polymer sample terminations were considered:positively charged(PAH-terminated)and negatively charged(PSS-terminated).Neutron reflectometry studies showed that,whereas positively charged samples did not favor the deposition of lipid,negatively charged samples allowed the deposition of a lipid bilayer with a thickness of approx5 nm.In the latter case,formation of polyelectrolyte layers after the deposition of the lipid layer was also possible.