BRAIN REGIONS INFLUENCED BY THE LATERAL PARABRACHIAL NUCLEUS IN ANGIOTENSIN II-INDUCED WATER INTAKE

摘要

This study examined which brain regions are influenced by an inhibitory lateral parabrachial nucleus (LPBN) mechanism that affects water intake. Controls and rats with bilateral LPBN lesions were administered angiotensin II (Angll) (0.5mg/kg subcutaneous - SC), drinking responses measured, and brains processed for Fos-immu-nohistochemistry. A separate group of LPBN-lesioned and non-lesioned animals were denied water for 90 min prior to perfusion to remove any confounding factor of water intake. LPBN-lesioned rats drank a cumulative volume of 9 mL compared with <4mL by controls (p < 0.01). Compared with sham-lesioned animals, Fos expression was attenuated in overdrinking LPBN-lesioned rats in the median preoptic nucleus (MnPO), paraventricular nucleus of the hypothala-mus (PVN), supraoptic nucleus (SON) (p < 0.001), bed nucleus of the stria terminalis and central nucleus of the amygdala (p < 0.01).