Major Histocompatibility Complex Genotyping with Massively Parallel Pyrosequencing

摘要

Pyrosequencing of complementary DNA-PCR amplicons was used to determine comprehensive major histocompatibility complex (MHC) class-I genotypes in primates that provide essential preclinical models for studies of infectious disease, vaccine development, and transplantation. By sequencebased typing of rhesus, cynomolgus, and pig-tailed macaques, more than 500 unique MHC class-I sequences were resolved, nearly half of which had not been reported previously. The approach showed remarkable sensitivity, demonstrating that pyrosequencing is a viable method for ultra-high-throughput MHC genotyping in primates and possibly in human beings.