Bidirectional effects of dexmedetomidine on human platelet functions in vitro

摘要

Platelets express the imidazoline (I)-receptor, I-1 and I-2, as well as the alpha(2)-adrenoceptor. Although dexmedetomidine, a selective alpha(2)-adrenoceptor agonist with some affinity for the 1-receptor is expected to affect platelet function, the effects of dexmedetomidine on platelet functions remain unclear. In the present study, we investigated the effects of dexmedetomidine on human platelet functions in vitro. The effects of dexmedetomidine on platelet aggregation were examined using aggregometers. The formation of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in platelets was measured by an enzyme immunoassay. In addition, P-selectin expression in platelets was estimated by flow cytometry. We showed that dexmedetomidine enhances platelet aggregation. But in the presence of yohimbine, an alpha(2)-antagonist, dexmedetomidine suppressed platelet aggregation. Efaroxan, an I-1-antagonist, and methylene blue, a soluble guanylate cyclase inhibitor, abolished the suppressive effect of dexmedetomidine, whereas idazoxan, an I-2-antagonist, showed no effect. Dexmedetomidine suppressed cAMP formation and enhanced P-selectin expression in platelets, and these effects were inhibited by yohimbine. Dexmedetomidine increased cGMP formation in platelets in the presence of yohimbine, and this increase was suppressed by efaroxan. These results demonstrated that dexmedetomidine has both enhancing and suppressive effects on human platelet functions through its action on the alpha(2)-adrenoceptor and on the I-1-imidazoline receptor, respectively. (C) 2015 Elsevier By. All rights reserved.