Naringenin suppresses the production of thymic stromal lymphopoietin through the blockade of RIP2 and caspase-1 signal cascade in mast cells.

摘要

Thymic stromal lymphopoietin (TSLP) plays a critical role in allergic diseases such as atopic dermatitis, asthma, and chronic obstructive pulmonary disease. Naringenin has various effects such as anti-atherogenic, anti-oxidant, and anti-inflammatory effects. However, the effect of naringenin on the production of TSLP has not been clarified. Thus, we investigated how naringenin inhibits the production of TSLP in the human mast cell line (HMC-1) cells. Naringenin inhibited the production and mRNA expression of TSLP in HMC-1 cells. The maximal inhibition rate of TSLP production by naringenin (100 muM) was 62.27 +/- 10.79%. Naringenin also inhibited the nuclear factor-kappaB luciferase activity induced by phorbol myristate acetate plus A23187. In the activated HMC-1 cells, the activations of receptor-interacting protein (RIP)2 and caspase-1 were increased, whereas the activations of RIP2 and caspase-1 were decreased by pretreatment with naringenin. These results suggest that naringenin can be used to treat inflammatory and atopic diseases through the inhibition of TSLP.