Simultaneous detection of metronidazole and chloramphenicol by differential pulse stripping voltammetry using a silver nanoparticles/sulfonate functionalized graphene modified glassy carbon electrode

摘要

A novel silver nanoparticles/sulfonated functionalized graphene modified glassy carbon electrode (AgNPs/SF-GR/GCE) was fabricated to determine chloramphenicol and metronidazole simultaneously. Taking advantage of sulfonic group, AgNPs were successfully electrodeposited on functionalized GR immobilized on the surface of a GCE. Scanning electron microscopy and energy spectrum analysis results confirmed that AgNPs were deposited on the functionalized GR film. Compared to the bare GCE or the pristine SF-GR modified electrode, AgNPs/SF-GR/GCE exhibited excellent electroreduction towards chloramphenicol and metronidazole. In addition, the two antibacterial drugs were separated completely in 0.10 M citric acid-sodium citrate buffer (pH 4.0) by differential pulse stripping voltammetry under optimum conditions. The cathodic current was linearly related with 0.02 similar to 20.0 mu M chloramphenicol and 0.10 similar to 20.0 mu M metronidazole, with the detection limits of 0.01 mu M and 0.05 mu M respectively. Furthermore, AgNPs/SF-GR/GCE was applied to the simultaneous determination of chloramphenicol and metronidazole in an aquatic product. (C) 2015 Elsevier Ltd. All rights reserved.