MagNA Pure LC DNA Isolation Kit III (Bacteria, Fungi) - Automated Isolation of Bacterial DNA form Various Sample Materials

摘要

The efficient isolation of bacterial DNA from different sample materials is crucial for fast and sensitive detection of microorganisms by polymerase chain reaction (PCR). Automation of such procedures is of tremendous help in the microbiology lab [1-5]. We therefore developed the new MagNA Pure LC DNA Isolation Kit III (Bacteria, Fungi) for the automated isolation of bacterial and fungal DNA from various types of research sample materials on the MagNA Pure LC Instrument. The standard protocol consists of very few manual steps (i.e., addition of a lysis buffer/proteinase cocktail and a heating step). All subsequent DNA isolation steps are automated on the MagNA Pure LC. The kit was tested with a variety of difficult sample materials like bronchoalveolar lavage (BAL), urine, sputum, stool, swabs, cerebrospinal fluid (SCF), tracheal secretion, blood and bacterial cultures. Samples were spiked with a known amount of certain Gram-positive and Gram-negative bacteria of with fungi, and the DNA was isolated and analyzed by species-specific PCR assays in the LightCycler (LC) Instrument [1-3].