The study of the possibility of gold hydrosol stabilization with nonthiolated homooligonucleotides with different lengths and sequences

摘要

The stabilization of citrate gold hydrosols with nonthiolated oligonucleotides having different lengths and sequences has been studied, with the oligonucleotides being added both at the stage of hydrosol synthesis (at 37 and 60 C) and after it. The oligonucleotides have been found to exhibit a pronounced stabilizing effect irrespective of the moment of their addition to the hydrosols. The only exception is the situation, in which adenine-based oligonucleotides are heated to 60 C in the course of the synthesis, when they, on the contrary, destabilize the hydrosols. Short-chain oligonucleotides (hexamers) exhibit a stronger stabilization effect than dodecamers do. Moreover, it has been shown that adenine-based and cytosine-based oligonucleotides show the strongest stabilizing effect.