Simultaneous Determination of Serotonin, β-Hexosaminidase and Histamine in Allergic Guinea Pigs Caused by Three Herbal Injections

摘要

The function of herbal injection is irreplaceable because of the quick action and definite therapeutic effects in the clinic, however allergic reactions frequently occurred and the fundamental research on its security is defective. In this study, in order to evaluate the allergenicity of Shuanghuanglian, Yinzhihuang and Danshen injection, in which two of them were often reported allergic reactions in the clinic and to explore the potential reasons of the allergenicity. Serotonin, β-hexosaminidase and histamine were used as the indexes to evaluate the allergenicity for the first time. Ovalbumin, chlorogenic acid and the three heberal injections were chosen to sensitize and challenge the guinea pigs. After administrated to stimulate the guinea pigs, concentrations of serotonin, P-hexosaminidase enzymes and histamine in Shuanghuanglian group, Yinzhihuang group and cholrogenic acid group were significantly increased compared with sodium chloride injection. However, there were no significant increases of three evaluated indexes in Danshen group. It is concluded that guinea pigs were more sensitized with Shuanghuanglian injection and Yinzhihuang injection which consists with the clinical medication. However, the sensitization of Danshen injection was very weak. The results have shown that using serotonin, β-hexosaminidase and histamine to evaluate the allergenicity of the herbal injection is reliable with high degree of accuracy. And cholrogenic acid might be one of the incentives of allergic reaction in Shuanghuanglian and Yinzhihuang injection.