Determination of Polygalacturonic Acid Content in Sweet Cherry (0900 Ziraat) Extracts by Fourier Transform Infrared Spectroscopy and Effects of CaCl2 and Ca(NO3)2 Foliar and Fruit Sprays Appications on Polygalacturonic Acid

摘要

Polygalacturonic acid (PGA) calibration standards were prepared by being mixed polygalacturonic acid with potassium bromide to cover a range of acid concentrations (10-98 %). A linear relationship between polygalacturonic acid content and carbonyl absorption band area was found (R~2 = 0.982). Polygalacturonic acid contents of 0900 Ziraat sweet cherry fruits were calculated from the linear fit equation. For fourteen different sources (1-14) of cherries, the polygalacturonic acid contents determined by FT-IR spectra were 16.68, 15.80, 65.56, 69.21,78.64, 74.10, 83.66,91.10, 53.46, 61.32, 48.19, 43.99, 39.62, 36.65 %, respectively. Foliar and fruits of cherry trees were sprayed with containing calcium chloride and calcium nitrate. Polygalacturonic acid content was affected by CaCh and Ca(NO3)2 applications and subsequently increased an average of 474 % with CaCl2 and 290 % with Ca(NO3)2 compared with the control.