Ultrasound Wave Assisted-Matrix Solid Phase Extraction for Determination of Synthetic Plant Growth Regulators Residues in Fruits and Vegetables by Ultra-High Performance Liquid Chromatography

摘要

An effective method using ultra-high performance liquid chromatography (UHPLC) was developed and optimized to obtain a complete separation of four representative synthetic plant growth regulators e.g., [2,4-dichlorophenoxyacetic acid (2,4-D), thidiazuron, forchlorfenuron and paclobutrazol] in fruits and vegetables. Extraction was performed with acetonitrile containing 0.5 % (v/v) acetic acid + 5 g sodium chloride (pH 4) under 3 min with an ultrasound wave assisted bath (40 kHz, 50 W), then 2 mL organic extraction and 1 g sorbents mixed. purified, analyzed by UHPLC and HPLC. The performance of chromatography was assessed by UHPLC and HPLC. Good recoveries and relative standard deviation were found for all analytes in fruits and vegetables.