Fluorimetric Determination of Trace Bisphenol-A Based on its Oxidative Reaction with Fenton-Like Reagent as Oxidant

摘要

Bisphenol-A (BPA) is an exogenous substance that acts like hormones in the endocrine system and disrupts the physiologic function of endogenous hormones. Due to the widespread human exposure to bisphenol-A and its potential adverse effects on human health, special attention has been paid to the determination of bisphenol-A in the environment. In this paper, a simple, sensitive and selective fluorimetric method for the determination of bisphenol-A, based on the oxidative reaction of bisphenol-A in the presence of hydrochloric acid by Fenton-like reagent (Fe~(5+)+H2O2), had been developed. The fluorescence came from the oxidation product of bisphenol-A at λ_(emission) 315 and λ_(emission) 415 nm in pH 8.90 Tris-HCl buffer solution. Under the optimum conditions, a linear relationship had been obtained between the fluorescence intensity and the concentration of bisphenol-A in the range of 0.01-0.5 μg/mL, with detection limit and correlation coefficient being 0.64 ng/mL and 0.9995, respectively. The proposed method had been successfully applied for the determination of trace bisphenol-A in water, beverage bottle and cling wrap film.