Applications of short-coherence digital holography in microscopy

摘要

We present an optical system based on short-coherence digital holography suitable for the imaging of three-dimensional microscopic objects. The short temporal coherence properties of the light source allow optical sectioning of the sample. Proper reconstruction of different layers within biological samples is possible up to a depth of a few hundred micrometers, but multiple scattering and inhomogeneities in the refractive index reduce the imaging quality for deeper layers. We have studied the possibility of numerically correcting sample-induced aberrations, and we now propose a method of improving image quality. Numerical simulations and preliminary experimental results show that compensation of these aberrations is possible to some extent. (c) 2005 Optical Society of America.