Microgel,nanogel and hydrogel-hydrogel semi-IPN composites for biomedical applications:synthesis and characterization

摘要

Quaternary ammonium salt hydrogels from a cationic monomer,(3-acrylamidopropyl)-trimethylam-monium chloride(APTMAC1),in a variety sizes such as bulk,micro-and nano-has been prepared.The synthesis of micro-and nanogels were carried out in the microenvironment of water-in-oil microemulsions using two types of surfactants,namely,L-alpha-phosphatidylcholine(lecithin)and di-octyl sulfosuccinate sodium salt(AOT).Additionally,hydrogel-hydrogel composite semi-interpenetrating polymer networks(semi-IPN)was synthesized by dispersing previously prepared microanogel into neutral monomers such as acrylamide(AAm)or 2-hydroxylethyl methcarylate(HEMA)before network formation.Hydrogel swelling and pH response behaviors have been investigated for bulk gels.Morphology,structure,and size of nano-,micro-and bulk materials were explored utilizing transmission electron microcopy(TEM),scanning electron microscopy(SEM)and atomic force microscopy(AFM).It was confirmed with gel electrophoresis that completely charged nanogel form a strong complex with DNA.