Determination of cyanide, in urine and gastric content, by electrospray ionization tandem mass spectrometry after direct flow injection of dicyanogold

摘要

A rapid and sensitive electrospray ionization tandem mass spectrometric (ESI-MS-MS) procedure was developed for the determination of cyanide (CN~-). CN~- in biological fluids was reacted with NaAuCl4 to produce dicyanogold, Au(CN)_2~-, which was extracted with methyl isobutyl ketone (MIBK). One microliter of the extract was injected directly into an ESI-MS-MS instrument. Quantification of CN~- was performed by selected reaction monitoring of the product ion CN~- at m/z 26 that derived from precursor ion Au(CN)_2~-at m/z 249. CN~- could be measured in the quantification range of 10~(-7) to 5 x 10~(-5) M with the limit of detection at 4 x 10~(-8) M using 10 μL of urine within 10 min. A victim's urine and gastric content were diluted with water to 4-fold and 500-fold and measured, respectively.