Preparation and preliminary evaluation of anhydrous tris(2,2 '-bipyridyl)ruthenium(III) perchlorate as a temporally stable reagent for analytical chemiluminescence

摘要

A new method for producing solutions of tris(2,2'-bipyridyl)ruthenium(III) ([Ru(bipy)(3)](3+)) which have significantly improved stability over other methods is presented and preliminary analytical evaluation of the reagent for chemiluminescence is reported. The synthesis of anhydrous tris(2,2'-bipyridyl)ruthenium(III) perchlorate ([Ru(bipy)(3)](ClO4)(3)) salt is described and the use of acetonitrile as solvent to minimise the presence of water which can be oxidised by [Ru(bipy)(3)](3+), which causes the inherent instability. The stability of the new reagent in acetonitrile was monitored spectrophotometrically (lambda(max) similar to 674 nm) and a decrease of <6% was observed. A flow injection analysis experiment was conducted using codeine to generate chemiluminescence to quantify the stability of the new reagent over 50 h. The results show a decrease in the chemiluminescence signal of <8%, which is a considerable improvement over previous methods. (C) 2000 Elsevier Science B.V. All rights reserved. [References: 17]