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A Fluorescent Chemodosimeter for Live-Cell Monitoring of Aqueous Sulfides

机译:用于活细胞监测硫化物水溶液的荧光化学计量仪

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Aqueous sulfides are emerging signaling agents implicated in various pathological and physiological processes. The development of sensitive and selective methods for the sensing of these sulfides is therefore very important. Herein, we report that the as-synthesized 1-oxo-1H-phenalene-2,3-dicarbonitrile (OPD) compound provides promising fluorescent properties and unique reactive properties toward aqueous sulfides. It was found that OPD showed high selectivity and sensitivity toward Na2S over thiols and other inorganic sulfur compounds through a sulfide involved reaction which was confirmed by high-resolution mass spectroscopy (HRMS) and nuclear magnetic resonance (NMR) results. The fluorescence intensity increases linearly with sulfide concentration in the range of 1.030 mu M with a limit of detection of 52 nM. This novel fluorescent probe was further exploited for the fluorescence imaging sensing of aqueous sulfide in HeLa cells.
机译:硫化水是与各种病理和生理过程有关的新兴信号传导剂。因此,开发敏感和选择性的方法来检测这些硫化物非常重要。本文中,我们报道了合成后的1-oxo-1H-phenalene-2,3-dicarbonitrile(OPD)化合物提供了有希望的荧光特性和对硫化氢的独特反应性。发现OPD通过与硫化物有关的反应,对硫醇和其他无机硫化合物显示出对Na2S的高选择性和敏感性,这通过高分辨率质谱(HRMS)和核磁共振(NMR)结果得到证实。荧光强度随硫化物浓度在1.030μM范围内线性增加,检出限为52 nM。该新型荧光探针被进一步用于HeLa细胞中硫化物水溶液的荧光成像传感。

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