The selective concentration of the contents in a microdroplet using spontaneous emulsification was proposed and demonstrated in a microfluidic channel. Aqueous microdroplets having a 40-mu m diameter, in octane containing 100 mM of Span 80, shrank to 10 mu m within 10 min with nanodroplet formation at the interface of the microdroplets. The microdroplets contents either stayed in the microdroplet or partitioned into the nanodroplets, depending on their properties. The size and the hydrophobicity of the contents are two parameters that determine concentration/separation. In addition, this method was applied to a bound complex and free ligand (B/F) separation to demonstrate its applicability to biochemical analyses. Here we report the separation of water-soluble molecules in microdroplets for the first tithe. This method is expected to enhance the flexibility of the design of droplet analytical processes and widen their applicability.
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机译：提出了使用自发乳化法选择性地浓缩微滴中内容物的方法，并在微流体通道中得到了证明。在含有100 mM Span 80的辛烷中，直径40微米的微滴在10分钟内收缩至10微米，并在微滴的界面处形成了纳米滴。取决于它们的性质，微滴内容物要么停留在微滴中，要么分配到纳米滴中。内容物的大小和疏水性是确定浓度/分离度的两个参数。此外，此方法应用于结合的复合物和游离配体（B / F）分离，以证明其在生化分析中的适用性。在这里，我们报告了第一个十分之一的微滴中水溶性分子的分离。该方法有望增强液滴分析工艺设计的灵活性，并扩大其适用性。
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