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Development of a Method to Extract and Purify Target Compounds from Medicinal Plants in a Single Step: Online Hyphenation of Expanded Bed Adsorption Chromatography and Countercurrent Chromatography

机译:一步从药用植物中提取和纯化目标化合物的方法的开发:膨胀床吸附色谱和逆流色谱的在线联用

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摘要

Pure compounds extracted and purified from natural sources are crucial to lead discovery and drug screening. This study presents a novel two-dimensional hyphenation of expanded bed adsorption chromatography (EBAC) and high-speed countercurrent chromatography (HSCCC) for extraction and purification of target compounds from medicinal plants in a single step. The EBAC and HSCCC were hyphenated via a six-port injection valve as an interface. Fractionation of ingredients of Salvia miltiorrhiza and Rhizoma coptidis was performed on the hyphenated system to verify its efficacy. Two compounds were harvested from Salvia miltiorrhiza, one was 52.9 mg of salvianolic acid B with an over 95% purity and the other was 2.1 mg of rosmarinic acid with a 74% purity. Another two components were purified from Rhizoma coptidis, one was 4.6 mg of coptisine with a 98% purity and one was 4.1 mg of berberine with a 82% purity. The processing time was nearly 50% that of the multistep method. The results indicate that the present method is a rapid and green way to harvest targets from medicinal plants in a single step.
机译:从天然资源中提取和纯化的纯化合物对于线索发现和药物筛选至关重要。这项研究提出了一种新型的二维联用膨胀床吸附色谱法(EBAC)和高速逆流色谱法(HSCCC),可在一个步骤中从药用植物中提取和纯化目标化合物。 EBAC和HSCCC通过一个六通进样阀连接起来。在联用系统上分离丹参和黄连的成分,以验证其功效。从丹参中收获了两种化合物,一种是纯度为95%的52.9 mg丹酚酸B,另一种是纯度为74%的迷迭香酸2.1 mg。从黄连中纯化出另外两种成分,一种是4.6毫克黄连碱,纯度为98%,另一种是4.1毫克黄连素,纯度为82%。处理时间几乎是多步法的50%。结果表明,本方法是一种从一步即可从药用植物中收获目标的快速而绿色的方法。

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