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Image-Based Single-Cell Sorting via Dual-Photopolymerized Microwell Arrays

机译:通过双光聚合微孔阵列的基于图像的单细胞分选

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We present a simple and cost-effective single-cell sorting method using two sequential photopolymerization steps that enables sorting based upon imaged phenotypes. The first photopolymerization step uses a thiolene-based resin with minimal autofluorescence to create an array of microwells to capture cells. The second photopolymerization uses (poly)ethylene glycol diacrylate to encapsulate undesired cells in a hydrogel, allowing for retrieval of the desired cell population using simple washing. We quantitatively characterize the method using fluorescently labeled cells and then applied the method to isolate cells based upon imaged fluorescence localization. The method is readily transferrable to other laboratories and will provide a facile route to sorting of cells based on imaged phenotypes.
机译:我们提出了一个简单且具有成本效益的单细胞分选方法,该方法使用两个连续的光聚合步骤,可以根据成像的表型进行分选。第一步光聚合步骤使用具有最小自发荧光的基于硫烯的树脂来创建微孔阵列,以捕获细胞。第二次光聚合使用(聚)乙二醇二丙烯酸酯将不需要的细胞封装在水凝胶中,从而允许通过简单的洗涤来回收所需的细胞群。我们使用荧光标记的细胞定量表征该方法,然后将其应用于基于成像荧光定位的分离细胞。该方法易于转移到其他实验室,并将为基于成像表型的细胞分选提供简便的途径。

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