Arrhenius Plot for a Reaction Catalyzed by a Single Molecule of beta-Galactosidase

摘要

The activity of a single enzyme molecule of Escherichia coli beta-galactosidase was measured using a capillary electrophoresis continuous flow assay. As the enzyme molecule traversed the capillary the incubation temperature was increased from 27 to 37 deg C, providing a continuous record of the change in rate with temperature. This data was used to develop a single enzyme molecule Arrhenius plot, from which the activation energy of the reaction was determined to be 31 kJ mol~(-1).