Membrane-Based Emitter for Coupling Microfluidics with Ultrasensitive Nanoelectrospray Ionization-Mass Spectrometry

摘要

An integrated poly(dimethylsiloxane) (PDMS) membrane-based microfluidic emitter for high-performance nanoelectrospray ionization mass spectrometry has been fabricated and evaluated. The approx100-(mu)m-thick emitter was created by cutting a PDMS membrane that protrudes beyond the bulk substrate. The reduced surface area at the emitter enhances the electric field and reduces wetting of the surface by the electrospray solvent. As such, the emitter enables highly stable electrosprays at flow rates as low as 10 nL/min and is compatible with electrospray solvents containing a large organic component (e.g., 90percent methanol). This approach enables facile emitter construction and provides excellent stability, reproducibility, and sensitivity as well as compatibility with multilayer soft lithography.