Surface Plasmon Resonance Biosensor Screening Method for Paralytic Shellfish Poisoning Toxins: A Pilot Interlaboratory Study

摘要

A surface plasmon resonance (SPR) optical biosensor method was developed for the detection of paralytic shellfish poisoning (PSP) toxins in shellfish. This application was transferred in the form of a prototype kit to seven laboratories using Biacore QSPR optical biosensor instrumentation for interlaboratory evaluation. Each laboratory received 20 shellfish samples across a range of species including blind duplicates for analysis. The samples consisted of 4 noncontaminated samples spiked in duplicate with a low level of PSP toxins (240 (mu)g STXdiHCl equivalents/kg), a high level of saxitoxin (825 (mu)g STXdiHCl/kg), 2 noncontaminated, and 14 naturally contaminated samples. All 7 participating laboratories completed the study, and HorRat values obtained were <1 demonstrating that the method performance was acceptable. Mean recoveries expressed as STXdiHCl equivalents/kg were 94.6 +- 16.8percent for the low level PSP toxin mix and 98.6 +- 5.6percent for the high level of saxitoxin. Relative standard deviations for within-laboratory variations (RSD_(r): repeatability) and between-laboratory variations (RSD_(R) velence reproducibility) ranged from 1.8 to 9.6percent and 2.9 to 18.3percent respectively. This first ever reported SPR biosensor interlaboratory study demonstrated this PSP application to be an empowering tool in the drive toward the reduction and replacement of the mouse bioassay within Europe.